LFS Lab Guide

Welcome

This site is a guide on the Policies and Procedures for working in the labs in the Faculty of Land and Food Systems. All personnel who will be conducting research in a laboratory environment, please go through all the information, complete the required forms if needed, and contact the responsible person assigned if you have any concerns or questions.

Getting Started

MacMillan Building

  • Monday - Friday: 7am - 7pm
  • Saturday, Sunday and Holidays: Closed

FNH Building

  • All building access doors must remain closed and secured at all times. If you are the last one to leave a lab or office space after office hours, you are responsible for securing all windows and doors, and re-arming the space.
  • Please do not prop open exterior doors or interior fire doors at any time.

For safety reasons, working alone after-hours (including weekends) is not recommended (as per WCB regulations). Due to the dangerous nature of some laboratory procedures and techniques, after-hours use of the laboratory chemicals and equipment is prohibited without explicit permission from both the faculty supervisor and the laboratory supervisor.

Refer to the UBC Risk Management Policy on Working Alone [pdf].

Some equipment is NOT to be used after hours due to safety regulation, listed below:

  • Macro Kjeldahl
  • All block digesters (macro, micro)
  • Bomb Calorimeter
  • Atomic Absorption Spectrophotometer
  • Fat Extractors
  • Furnaces

Consult your supervisor if you require access into building after hours.

Access through FOB-designate entrances only.

All building access doors should be closed and secured at all times.

Note: The Last One to leave a lab or office space after 7 pm are responsible for securing all windows and door, and rearming the space. Please do NOT prop open exterior doors at any time.

All labs and offices will be alarmed outside of regular building hours.

LFS Lab/Office Secure Access Code Request

LFS Key Requests

Keep all laboratory access doors closed and shut at all times

Note: Working alone in the laboratory after-hours and on weekends is NOT RECOMMENDED (as per WCB regulations).

Lab Safety Orientation

Please review the LFS Minimum Safety Training Recommendations for all Workers as a guideline of the training you may be required to take.

All undergraduate and graduate students are required to complete one of the two safety orientations:

As part of your lab orientation, please familiarize yourself with location of the nearest emergency equipment and first aid kit before you begin your lab shift.

MacMillan Building, Locations of:

  • Nearest Exit Doors: some doors direct to other rooms, Not the Exit. Follow the exit route posted on corridor's walls or follow the nearest exit sign.
  • First Aid Kits: All labs, the Dean's Office (MCML Rm 248), Student Services Office (MCML Rm 344), and the Learning Centre (MCML Rm 264)
  • Safety Showers: MCML Rm 102, 112, 118, 120, 148, 202, 218, 230, 240, 302A, 320, 328, and 336.
  • Eye Wash Stations: MCML Rm 102, 112, 118, 120, 130, 136, 148, 202, 218, 230, 240, 302A, 320, 328, and 336.
  • Fire Extinguishers: Every lab and hallway. (Be aware of the different types of extinguishers and their uses.)
  • Material Safety Data Sheets (MSDS): Online.
  • Chemical Spill Treatment Kits: MCML Rm 112, 202, 214, 240, 302A, and 336.

FNH Building, Locations of:

  • First Aid kits, eye wash stations, safety showers and chemical spill treatment kits  are located in all FNH Labs
  • Fire extinguishers are located in every lab and hallway. Be aware of the different types of extinguishers and their uses.
  • Material Safety Data Sheets (MSDS) are located in FNH 110 or Online.

For life-threatening emergencies, call 9-1-1

Please call UBC Campus Security 604-822-2222 if you have any safety concerns or questions.

  • Fire: PULL the nearest FIRE ALARM. Close the doors behind you as you exit a room. Follow the nearest exit route, and then CALL 9-1-1. Give them the full address of the building (2357 Main Mall for MCML, or 2205 East Mall for FNH), and the room number where the fire began.
    • MCML: The meeting point is across Main Mall, between MacLeod and the Computer Science buildings. Remain at your designated meeting location until everyone has been accounted for.
    • FNH: Meet across the street on East Mall at the School of Population and Public Health. Remain at your designated meeting location until everyone has been accounted for.
  • Gas Leak: The same procedure as in a fire should be followed except you should try to close the gas valve in your laboratory if it is accessible. Report the source/room of the leak to Lesley/Pedro (MCML) or Peter/Patrick (FNH).
  • Major Spills: Evacuate the affected area. Close the door behind you. Call 9-1-1.
  • Work Related Injuries (WCB): All work-related injuries must be reported to Lesley Dampier, the Safety Committee Chair, as soon as possible after immediate first aid has been given. See Risk Management for a list of WCB and other accident reporting forms.
    • W.C.B Coverage: Personnel who are not on UBC Payroll (students, visitors) are NOT covered by WCB and should liaise with the HR Director for information on obtaining additional coverage.
  • Working Alone: For safety reasons, it is NOT RECOMMENDED to work alone in the laboratory after-hours including weekends (WCB regulations).

Lab Regulations

Important Contacts

Safety Guidelines

Personal Protective Equipment (PPE)

  • Lab Coats
  • Goggles
  • Face Shields
  • Masks
  • Rubber Gloves
  • Oven Mitts
  • Aprons

Proper Use/Storage of Chemicals/Materials

  • Severe Poisons, Carcinogens and Suspected carcinogens
  • Radioactive Material
  • Living Organisms, and especially for FNH users
  • Acids & Bases
  • Flammable Liquids
  • Compressed Gas Cylinders
  • Chemicals of Special Note:
    Benzene, Carbon Tetrachloride, Mercury compounds, Nitric acid, Organic nitro compounds, Perchloric acid, and Picric acid.

Proper Use of Equipment

  • Fumehoods
  • Electrical/Plumbing
  • Damaged or Non-Functioning Equipment

Working Alone

Chemical Purchasing

Chemical Deliveries

Waste Disposal Management

  • Hazardous Waste Management Information Sheet (HWMIS)
  • Broken Glass Waste
  • Glass Bottles Not Broken
  • Plastic Waste
  • Needles, Pasteur Pipettes and Other Sharp Objects
  • Chemical Disposal
    • Liquid Organic Chemical Waste
    • Solid Organic and Inorganic Chemicals
    • Water-Soluble Non-Hazardous Chemicals
  • Biomedical Waste
  • Pathological Waste

Common Equipment Available For All

Common Chemicals For Use

Videos

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MacMillan Building

Lesley Dampier

MCML Rm 125

ph:604-822-4877

cl:604-816-5641

lesley.dampier@ubc.ca

Pedro Aloise

MCML Rm 22A

ph:604-822-3488

cl:604-219-6728

pedro.aloise@ubc.ca

Sylvia Leung

MCML Rm 208

ph:604-822-3454

sylvia.leung@ubc.ca

For more contacts information, please refer to https://my.landfood.ubc.ca/health-safety/Important Contacts.

FNH Building

Peter Hoffman

FNH Rm 110B

ph:604-822-4601

peter.hoffman@ubc.ca

Patrick Leung

FNH Rm 230

ph:604-822-2793

patrick.leung@ubc.ca

For more contacts information, please refer to https://my.landfood.ubc.ca/health-safety/Important Contacts.

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  • Laboratory coats and solid shoes (not sandals) must be worn at all times when working with chemicals.
  • Rubber gloves and plastic goggles must be worn when working with acids, bases, or poisonous chemicals.
  • All laboratory chemicals should be regarded as poisons. The mouth should never be used in pipetting.
  • All operations involving the risk of irritating or toxic vapor must be carried out in a fumehood with adequate ventilation.
  • Filter paper, broken glass and other solids must not be disposed of in sinks. Special metal bins are located throughout the laboratory for the disposal of glass waste. Separate plastic containers must be used for disposal of sharps and needles. These are also located throughout the lab. Please do NOT use the glass waste bins for paper waste.
  • Strong acids and bases must be collected and sent to the Chemical Waste Disposal Facility at South Campus for neutralization and disposal. See Dept. Technicians for proper processing procedure prior to pick-up of chemicals by CWDF drivers.
  • Bunsen burners should only be turned on while in use, and should be turned off immediately afterwards. Flammable volatile fluids must NOT be placed near any flame source.
  • Location of fire extinguishers, first aid kits, safety shower, and eyewash stations must be known before initiating any procedure entailing the use of chemicals or equipment.
  • Smoking, eating, or drinking is not permitted in the laboratory.
  • All accidents must be reported. Even apparently minor injuries can develop serious side effects. Immediate treatment is essential. If somebody is seriously injured, call 911, explain the nature of the injuries, and give location with address and room number. For MCML, report any accident to Lesley Dampier (lesley.dampier@ubc.ca). For FNH, report to the First Aid Attendants Peter (2-4601)/Patrick (2-2793). Remember afterwards to fill out an accident report for WCB. An Incident/Accident Report MUST be completed and submitted to UBC Risk Management for all incidences. Failure to do so may invalidate workers’ compensation claims. Incident/Accident Reports can be downloaded for filling out from the UBC Risk Management website: http://riskmanagement.ubc.ca/z-forms-and-publications
  • Ensure you clearly understand the hazards involved before commencing any laboratory procedure. Be especially aware of the toxic nature of the chemical compounds you will be working with. If in doubt, ask.

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Each lab/PI have their own PPE when required for each type of protection.

  • Lab Coats
    • Cotton only, no polyester or any flammable fabric allowed.
    • Must be worn while working in labs (users are required to provide their own).
    • Absolutely NO shorts or short dresses, and NO open-toed shoes are permitted in the labs.
  • Goggles
    • Goggles/eye protection is mandatory when working with acids, bases, flammable liquids, and hazardous chemicals.
    • Must be worn while working in labs.
  • Face Shields
    • Must be worn while working with flying objects, chemical splashes, or potentially infectious fluid in labs.
  • Masks
    • Dust masks must be worn while working with DUST in labs.
  • Respirators:
  • Rubber Gloves (disposable gloves)
    • Must be worn while working in labs.
  • Oven Mitts
    • Beside each oven.
    • Ask if not found.
  • Aprons
    • Ask if needed.

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Please refer to UBC Laboratory Chemical Safety Manual for more information:http://www.riskmanagement.ubc.ca/health-safety/chemical-safety/chemical-safety-manual

Prior to using any chemicals for the first time, you must consult the Material Safety Data Sheets (MSDS). Material Safety Data Sheets are located online (http://riskmanagement.ubc.ca/health-safety/chemical-safety/materials-safety-data-sheets) or FNH Rm 110. These information sheets contain valuable information pertaining to the safe handling, storage, incompatibility and disposal of the chemical in question. Make sure you have read and fully understood all the hazards associated with the chemical. As an exercise, look up “acetone”.

Note: Users are responsible to keep track of their current chemical inventory.  

 

Severe Poisons, Carcinogens and Suspected Carcinogens

MCML Building:
  • These chemicals may not be stored in the laboratory longer than the time necessary for their intended use. Once an experiment utilizing these compounds is complete, the chemicals must be disposed of in a safe and proper manner. For disposal information, see following Waste Disposal Management section for chemical disposal.
  • Any of these compounds to be used over longer periods of time must be labelled and stored correctly.
  • Prospective users of these compounds must report to their PI or supervisor for appropriate instruction in the safe handling and disposal of such compounds.
FNH Building:
  • These chemicals may not be stored in the laboratory longer than the time necessary for their intended use. Once an experiment utilizing these compounds is complete, the chemicals must be disposed of in a safe and proper manner. For disposal information, consult the Head Technician.
  • Any of these compounds to be used over longer periods of time must be labelled and stored correctly. The designated storage areas are located at the Solvent Cabinet of FNH Rm 395 for Carcinogens.
  • Prospective users of these compounds must report to the Head Technician for appropriate instruction in the safe handling and disposal of such compounds.

 

Radioactive Material

As per UBC Policy, anyone working with radioactive material in any UBC lab, must first complete a recognized course in the safe handling and disposal of radionuclides. (See http://riskmanagement.ubc.ca/health-safety/radiation-safety).

It is the responsibility of the Radioisotope Licence Holders and their authorized users to ensure all proper procedures and practices are followed as outlined by the UBC Radiation Safety Program.

MCML Building:
  • Radiation Safety Purchasing System: http://riskmanagement.ubc.ca/health-safety/radiation-safety/purchasing
  • Prospective users of radioactive material must understand and follow all appropriate instruction in the safe handling, storage, and disposal of such material.
  • Radioactive material may only be used and stored in designated labs that have been certified as radioisotope rooms, and licensed under the authorized user(s). Radioactive material may NOT be stored in any other location.
  • Radioactive material may only be stored in the following locations certified by UBC Radiation Safety Program:
    • MCML Building: Refrigerator in Rm 220A
FNH Building:
  • Prospective users of radioactive material must report to the Head Technician for appropriate instruction in the safe handling, storage, and disposal of such material.
  • Radioactive material may only be stored in the following locations certified by UBC Radiation Safety Program:
    • FNH Building: Please consult with Peter.

Radioactive materials may NOT be stored in any other location.

 

Living Organisms

  • All items contaminated with living organisms must be sterilized in the autoclave. All spills of living organisms must be disinfected with Wescodyne immediately. Benches and biological safety cabinets must be decontaminated after working with living organisms.
  • Use the red Biological Waste Disposal Tags for disposing of biological waste, remember to check the appropriate box (autoclaved risk group 1, risk 2, anatomical waste, animal waste, etc) and affix a generator barcode. (You can obtain generator barcodes from Risk Management by emailing your request to Bang Dang, bang.dang@ubc.ca. Include the following info in your email: Building Name, Faculty/Dept Name, your name, room number, and telephone number).
  • Ensure proper autoclave procedures are followed, as outlined on the Treatment & Disposal of Biohazardous Waste.
  • Especially for FNH users:
    • Use propipette when transferring cultures. Contact Dr. Kevin Allen (FNH 218, kevin.allen@ubc.ca) for further advice.
    • Put at the back door cabinet of FNH building on Tuesdays before 9:30 am for Environmental services to pick up. Check if the material was picked up by the end of the day. If it has not been picked up return it to your lab cold room until the next pick up day (do not leave biohazards in the hallway overnight). If you have any inquiries about disposing of biohazard waste please see Peter or phone at 2-8132.
    • Make sure you use the correct color autoclave bags for the type of waste you are generating (risk type 1 / 2). Also once the bag cools down double bag your waste using a clear regular plastic bag so that it does not leak when they come to pick it up.

 

Acids & Bases

 

Flammable Liquids

  • The maximum container capacity for any flammable liquid is 4.5 L. maximum of 25 L of flammable liquid is allowed to be stored in per lab (i.e. 5 x 4.5 litres per lab).
    • For MCML, any additional quantities must be stored in the Solvent/Chemical Storage Room (basement of MacMillan, room 56). Obtain the key from Sylvia Leung, MCML Room 208.
  • All flammable liquids must be stored in the appropriate flammable storage cabinet. Only flammables may be stored in these cabinets.
  • When using flammable liquids, only use small quantity which may be present in your work area. Transfer the approximate amount of flammable liquid needed to a smaller container inside a fumehood, then return the stock container to the storage cabinet. Under no circumstances will the presence of more than 25L of flammable liquids be allowed in a lab.
  • NO flammable liquids may be stored in refrigerators. Cooling flammable liquids rarely reduces their volatility and storing these liquids in refrigerators may result in an explosion.
  • Certain flammable liquids form explosive peroxides once the stock container has been opened. Examples of these are:
    • Diethyl or Ethyl Ether
    • Dimethoxyethane
    • Dioxane
    • 2-Ethoxyethanol
    • Ethylene Glycol Monoethyl Ether
    • Isopropyl Ether -> This is a severe peroxide hazard.
    • 2-Methoxyethanol
    • Tetrahydrofuran

(This is NOT a complete list!!)

This liquids should be checked for peroxide build up prior to using them. Any liquid with unsafe peroxide levels must be disposed of with extreme care.

 

Compressed Gas Cylinders

  • Gas cylinders must never be left unchained.
  • Never tamper with safety valves.
  • Cylinder valves must be properly capped when moving tanks. Use the designated cylinder cart to transport all gas tanks.

 

Chemicals of Speical Notes

  1. Benzene - This should only be used if there is no other solvent which can be substituted for it. it must be handled with extreme caution as over 25 ppm concentration (far below the level detectable by the nose) will result in a serious health risk.
  2. Carbon Tetrachloride - Concentrations greater than 10 ppm result in liver damage. Use with extreme care.
  3. Mercury compounds - All Mercury compounds are severe poisons. They are not permitted (including thermometers) in the Food Grade Labs, FNH 130, 140 and 190.
  4. Nitric acid - Gaseous oxides of NO2 are dark brown and upon inhalation cause edema which may lead to death.
  5. Organic nitro compounds - e.g. Aniline, Nitrobenzene. Any contact through skin or breathing results in cyanosis (insufficient blood oxygenation.)
  6. Perchloric acid - Digestions or other forms of heating perchloric acid are not allowed. Extreme care must be taken when storing and handling this acid as any spill will result in the production of anhydrous perchlorates which will explode with friction.
  7. Picric acid - Picric acid must not be allowed to dry out. Anyone wishing to use picric acid must maintain this chemical completely submerged in water. Picric acid should be stored in a cool, dry place but not in a refrigerator.

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Please refer to UBC Laboratory Chemical Safety Manual for more information:http://www.riskmanagement.ubc.ca/health-safety/chemical-safety/chemical-safety-manual

  • Fumehoods
    • Follow all instructions posted on fume hood sashes.
    • Ensure proper personal protection equipment are used when working in fumehoods.
  • Electrical/Plumbing
    • If you see a major electrical problem report to Pedro/Lesley (MCML) or Peter/Patrick (FNH) or notify UBC Trouble Calls at 604-822-2173. Water and electrical equipment can be a dangerous combination, wipe up any water spills inside fume hoods when electrical equipment is being used.
  • Damaged or Non-Functioning Equipment
    • Please report instrument malfunction immediately to Lesley (MCML) or Peter (FNH).

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For safety reasons, it is NOT RECOMMENDED to work alone in the laboratory after-hours including weekends (WCB regulations). Refer to theUBC Risk Management Policy on Working Alone [pdf] or http://riskmanagement.ubc.ca/health-safety/working-alone.

According to UBC Risk Management, it is not recommended to work alone in the laboratory unless get permitted by individual's PI. The definition of working alone is, “the performance of work by an individual who is out of audio or visual range of another individual for more than a few minutes at a time”.

The other individual must be aware of the safety aspects of this laboratory and also be familiar with the emergency procedures. If it is not sufficient to rely on an individual otherwise occupied in another area of the laboratory, the other individual must be in the work area and be able to provide immediate aid.

Even when performing relatively safe procedures, at least one other person in the laboratory should be aware that you are working alone, of the nature of the work you are doing, and how long you will be performing the task. Checks at half-hour intervals will reduce the risk of a serious injury going unnoticed.

  • From the CRC Handbook of Laboratory Safety:

“No Wet digestion or otherwise hazardous procedures may be performed in this laboratory outside regular working hours of 8:30 am to 4:30 pm weekdays.”

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Observe any warnings when deciding on the quantity of chemical to order. If the chemical is of concern, consult with your supervisor/PI (MCML) or the Head Technician (FNH) prior to placing your order.

When ordering flammable liquid, it is suggested that you order smaller batches. While this may be more costly, it will make the handling and transfer of the material safer, as well as minimizing the costs associated with disposal of any excess chemicals.

  • Some flammable liquids for explosive peroxides during storage, once its container has been opened.

MacMillan Building

FNH Building

  • All orders for chemicals, whether for student labs or research purposes, must follow the LFS Purchasing site (https://lfs-purchasing.sites.olt.ubc.ca/)
  • Prior to placing orders, consult the MSDS located in FNH 110 or online (riskmanagement.ubc.ca/health-safety/chemical-safety/materials-safety-data-sheets) to determine any special hazard warning.
  • If the MSDS for the specific substance is not available, consult both Hazards in the Chemical Laboratory (edited by Bretherick) and the Aldrich Chemical Company's Catalog Handbook of Fine Chemicals (both books are located on top of the MSDS filing cabinet).

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  • All chemicals entering the Laboratory, whether through delivery or borrowing from another Department, must be provided with a label stating the date of receipt, user, and required date of disposal (if applicable).
  • All hazardous chemicals must have a sticker attached indicating the nature and degree of danger. At no time should these stickers be removed from the dangerous chemical.

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Introduction to Hazardous Waste Management at UBC

 

Hazardous Waste Management at UBC: Repeated Waste Streams: Biomedical, Non-Regulated Contaminated Solid, Solvent/Flammable Liquid, Photochemical Wastes

 

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Hazardous Waste Management Information Sheet (HWMIS)

Please check the overall Waste Disposal Guide and the procedure of Chemical Waste Disposal for more information. Information concerning the disposal of chemical waste can be found in the Aldrich Chemical Company's Catalogue Handbook of Fine Chemicals and on the MSDS.

(NEVER dispose glassware, sharps, plastics / biomedical waste, unused chemicals / chemicals waste and pathological waste directly to the garbage bins, sinks, or drains.)

Any enquiries regarding the disposal of chemicals should be directed to:

MCML: Lesley Dampier, Pedro Aloise, or Sylvia Leung.

FNH: Peter Hoffman, Patrick Leung 

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MCML

IMG_8536 IMG_8537

FNH

Broken Glass Waste

  1. Collect in 5 gal. gray metal pails lined with plastic bags, marked “GLASS WASTE ONLY”. Cardboard boxes are not acceptable.
  2. Do NOT fill more than ¾ full (no glass is to protrude past top of can).
  3. When ¾ full, choke off and tie with plastic cable tie.
  4. Label with tag stating Department, Room number, Name & Phone number.
  5. Take pails to the building’s designated location for waste pickup: MacMillan - basement loading dock. FNH - back door cabinet of FNH building on Tuesdays before 9:30 am for Environmental services to pick up.
  6. New pails will be exchange when pails are picked up. Please remember to take new empty pails back to your lab.
  7. NO PAPER, PLASTIC, NEEDLES, SYRINGES IN THESE CANS, BROKEN GLASS ONLY!

Glass Bottles NOT Broken (e.g. Winchester bottles, reagent bottles.)

  1. Rinse empty bottles thoroughly with cold water.
  2. Discard the caps in regular garbage bin.
  3. Place bottles in a cardboard box lined with clear plastic bag, labeled “GLASS BOTTLES FOR DISPOSAL”.
  4. Take boxes to the building’s designated location for waste pickup: MacMillan - basement loading dock.

Plastic Waste (e.g. clean plastic bottles)

  1. Collect plastic bottles in plastic bags and then package them into sturdy cardboard boxes.
  2. Mark the boxes “PLASTIC WASTE FOR INCINERATION” indicating your name and phone number.
  3. Take boxes to the building’s designated location for waste pickup: MacMillan - basement loading dock.

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MCML

IMG_8536 IMG_8537

FNH

Needles, Pasteur Pipettes and Other Sharp Objects

  1. Dispose in plastic Red/Yellow cans/jugs marked “USED SHARPS, NEEDLES, PASTEUR PIPETTES FOR DISPOSAL” (usually found on bench-tops scattered around the labs.)
  2. When container is full, securely close lid.
  3. Affix Red Biological Waste Disposal tag and attach generator barcode sticker on tag.
  4. Take container(s) to the building’s designated location for waste pickup: MacMillan - basement loading dock, “Chemical Waste Disposal Cages”. Obtain keys from Sylvia (room 208).

Chemical Disposal

Information concerning the disposal of chemical waste can be found in the UBC Laboratory Chemical Safety Manualand on the MSDS.

Hazardous Waste Management at UBC: Chemical Waste Packaging Materials

 

Hazardous Waste Management at UBC: Chemical Waste Inventory System

 

Liquid Organic Chemical Waste

  • Use Red solvent disposal cans. Partially filled cans should be kept in a fumehood.
  • Affix Blue Flammable Liquid tag and attach generator barcode sticker on tag.
  • Do Not mix halogenated and non-halogenated compounds in the same disposal can. If you are in doubt as to the nature of the substance you have, do not add it to a can already containing chemicals. Instead, place it in a separate can, and label it “UNKNOWN CONTENT”.
  • Once full, these cans are to be taken to the building’s designated location for waste pickup: MacMillan - basement loading dock, “Chemical Waste Disposal Cages”. Obtain keys from Sylvia (room 208). Remember to fill out the Blue section of the tag with identified chemical(s) attached to the can. (DO NOT leave filled cans in the same area as the empty cans).
  • New cans will be exchange when full cans are picked up. Please remember to take new empty cans back to your lab.
  • For other chemicals make sure bottles are labeled with the correct chemical(s) contained.
  • NEVER DISPOSE OF ORGANIC CHEMICALS IN THE SINK.

Solid Organic and Inorganic Chemicals

  • Identify the chemical to be disposed of. Label it, or better still, leave it in its original container. Consult Waste Disposal Guide (MCML) or Dept. Technicians/Head Technician (FNH) for proper disposal procedure.

Water-Soluble Non-Hazardous Chemicals

  • Run water into sink for at least one minute to minimizes chances of there being a reactant present in the drain.
  • Dilute the chemical to be disposed of with copious amounts of water, then pour it down the drain. Allow the water to run for at least five minutes afterwards to prevent a chemical reaction from occurring in the plumbing.

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MCML

IMG_8536 IMG_8537

FNH

Biomedical Waste (e.g. Tissue culture flasks/plates, plastic petri dishes, syringes, pipettes tips, etc.)

  1. Autoclave or decontaminate before discarding.
  2. Check Biohazardous Risk Group, Collect in appropriate waste bags (Clear, Red or Black), and Double bag to prevent leaking. Do not exceed 10 Kg.
  3. Affix Red Biological Waste Disposal tag and attach generator barcode sticker on tag. Indicate contents of bag (e.g. plastic culture plates.)
  4. Choke off bag and tie with tag attached.
  5. Take bag(s) to the building’s designated location for waste pickup: MacMillan - basement loading dock, “Biological Waste Only Room”. Obtain keys from Sylvia (room 208) and fill out log book.
  • Exception: If the samples may become smelly/mouldy/rotten/running, take bag(s) to the building’s designated location for waste pickup: MacMillan - basement loading dock, “Pathological Waste Freezer”. Obtain keys from Sylvia (room 208) and fill out log book.
  • For FNH Users, put at the back door cabinet of FNH building on Tuesdays for Environmental services to pick up. Check if the material was picked up by the end of the day. If it has not been picked up return it to your lab cold room until the next pick up day (do not leave biohazards in the hallway overnight). If you have any inquiries about disposing of biohazard waste please see Peter or phone at 2-8132. Make sure you use the correct color autoclave bags for the type of waste you are generating (risk type 1 / 2). Also once the bag cools down double bag your waste using a clear regular plastic bag so that it does not leak when they come to pick it up.

NEW Disposal procedures for risk groups 1 & 2 - Autoclave room responsible person

 

New Disposal Procedures for Lab Users - Autoclave solid waste w/ RG 1 & 2 contaminated materials

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MCML

IMG_8536 IMG_8537

FNH

Pathological Waste (e.g. dead animals, tissue samples, biological specimens)

  1. Package in a Black 6 mil polypropylene bags and Double bag to prevent leaking. Do not exceed 10 Kg.
  2. Affix Red Biological Waste Disposal tag and attach generator barcode sticker on tag. Indicate contents of bag (e.g. dead chickens.)
  3. Choke off bag and tie with tag attached.
  4. Take bag(s) to the building’s designated location for waste pickup: MacMillan - basement loading dock, “Pathological Waste Freezer”. Obtain keys from Sylvia (room 208) and fill out log book.

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MacMillan Building

(Please keep clean for all usage)

  • Ice machine (Rm 302)
  • Water distillations (Rm218, Rm230)
  • Superspeed Centrifuge (Rm230): please check with Estelle (Rm 230 C) before use.
  • 2 Autoclaves (portable and stationary) (Rm302)
  • Walk-in cold room (Rm230)
  • Dry oven (Rm218)
  • Chest freezer (Rm220): for temporary storage ONLY.

FNH Building

(Please keep clean for all usage)

Walk-in Coolers and Freezers (Rm193-194, Rm140):

  • There are two walk in coolers (5-7°C) and two freezers (-25-30°C) in the ground level that are classified as Food Grade. (No chemicals, no hazardous materials, no bio-hazards or radio-chemicals allowed). If you have doubts about a food grade area ask Peter.
  • The freezer door has an emergency button on the inside that you push if the door closes on you while working inside.
  • The safest thing to do would be to notify a co-worker or supervisor that you will be going into the walk in freezer. Also there is a block of wood on the floor right next to the door to keep the freezer door ajar. In case the block is not there use a chair or anything big to keep the door ajar.
  • Take special care when going into the freezer while you are here alone.

FNH 190 (Food Preparation Area), FNH 140 (Taste Panel) and FNH 130 (Vij's Kitchen):

  • These rooms have utensils and glassware that are used only for food preparation. Do Not bring any utensils from the food preparation areas to the laboratories. Also, do Not bring any contaminated glassware, chemicals, petri dishes, mercury thermometers from the analytical laboratory areas into rooms 140, 130 and 190. Since some of the food prepared in these rooms will be consumed by people, we are trying to avoid any cross contamination of food with hazardous materials from the analytical labs.
  • If by mistake you bring a piece of glassware from rooms 190, 130 or 140 to the analytical laboratory areas, leave it up in the lab, as it is considered contaminated material and should not be returned to 190, 130 or 140.
  • Beginning in the Spring of 2010 students using the food grade areas will have to wear marked lab coats that will be available for work only inside food processing areas. Do not use these food grade lab coats in the laboratory areas upstairs at all! Likewise do not use any lab coat that you have been wearing in the analytical labs in any food grade areas. This is to prevent any cross contamination of our food grade areas with biological or chemical hazards from the labs above. In addition you will have to wear hairnets in food grade areas.

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MacMillan Building

Chemicals freely available for research and teaching are located in Room 220 (on the shelves and down cabinets). Please check with Sylvia prior to taking from the shelf. Please return any unused portions as soon as possible, and notify Sylvia if any chemicals are used up to ensure a current inventory may be maintained.

Please Log all usage in Logbook, and Close shelf doors after taking or returning chemicals.

Please return chemicals to its proper Alphabetical order and Classification.

  • Excess chemicals (newer than year 2000, and in good and usable condition only) from PI can be donated and added to the cache.
  • Please check with to Sylvia Leung (Rm208) before donation.

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Safety Rules

Safety Rules for Chemistry Lab Necessary Safety Rules for Chemistry Lab Video

WHIMS/GHS

WHMIS - Overview (1 of 5). For more information, please refer to Work Safe BC

MSDS

MSDS & SDS

Personal Protection Equipment (PPE)

Proper Dress and PPE / Lab Safety VideoSafety Rules for Chemistry Lab Necessary Safety Rules for Chemistry Lab Video Part 1.

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Analytical Balance

  • Quick Review: 11 steps introduction on How-to-weigh

Laboratory Technique - Analytical Balance

  • Weighing/Tare/Weighing by Difference

Weighing Using An Analytical Balance

  • Sample handling/Set up/Safety Precautions/Errors Discussion

Analytical Balance

  • Calibration

Calibration of an Analytical Balance

Gas Cylinders

  • Transportation

The Basics of Cylinder Handling

  • Regulator Installation

Regulator Installation

Microscopes

  • Compound Microscope

Quick Review: Set up & Clean up Compound Microscopes with troubleshooting solution

Detailed on How to Use Compound Microscopes

  • Oil-immersion lens on Compound Microscope

Oil Immersion

  • Stereo Microscope

    How to Use Stereo Microscopes

  • Tri-nocular Microscope Camera

pH Meter

  • Stationary pH meter

Using a pH Meter

  • Handheld pH meter
  • Earlier-model Stationary pH meter

How to Use a pH Meter

Volumetric Pipetting

  • Pipetting with ball

TD (to deliver) VS. TC (to contain) Pipet

Safety Bulb VS. ASE Bulb

  • Pipetting with pump (two types: I & II)

Pipet Pump I

Pipet Pump II

  • Variety of pipetting

Using Serological Pipets

  • Cleaning a Volumetric Pipet

BHC Cleaning a Volumetric Pipet

  • Micropipetting

Micropipetting

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Dilution

How to Prepare a Dilute Acid Solution

Graphing

  • Graphing by Hand

Graphing Data by Hand

  • Graphing by Spreadsheet

Graphing Data by Spreadsheet

  • Semi-log graphing by hand

Semilog Graph Paper

  • Semi-log graphing by Excel

Log graphing in Excel

Significant Figures (sig. fig.) Rules

  • Zeros

Significant Figures - Rules made easy

  • Addition and Subtraction

Significant Figures - Adding and Subtracting

  • Multiplication and Division

Multiplication and Division with Significant Zeros

  • Scientific Notation

Scientific Notation and Significant Figures, Part I

Scientific Notation and Significant Figures, Part II

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Additional Resources

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